Composite

Part:BBa_K145267

Designed by: Nathalie Busschaert   Group: iGEM08_KULeuven   (2008-09-07)


luxR generator + GFP under control of luxR

GFP production is controlled by a promoter repressible by luxR and c2 P22. LuxR is constitutively generated, so normally GFP production is switched off. By addition of HSL luxR can be deactivated. In that case GFP will be generated.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 985
    Illegal BsaI.rc site found at 1724


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